Concentration Test for Ferrous Sulfate (FeSO4) and Thiamin in Murashige and Skoog Medium on The Orchid Sub-Culture Dendrobium SP By In-Vitro

The Dendrobium orchid is one type with special features and many variations. This study aims to determine the administration of various concentrations ferrous sulfate (FeSO4) Thiamin sp explants on Murashige And Skoog media. design used in this was a Factorial Completely Randomized Design (CRD) consisting 2 treatment levels (F = FeSO4 T Thiamin) three replications. Namely: F0 (Without FeSO4), F1 (FeSO4 26.8 mg/l ), F2 27.8 mg/l), F3 28.8 T0 Thiamin), T1 (Thiamin 0.1 T2 0.2 mg/l) l), T3 0.3 mg/l). Based results, by giving had single effect shoots quantity parameters best found an average (.03 ea), for shoot height (1.37 cm), total leaves (6.14), roots (5.17), root length were 1.29 cm sp. Orchid explants. For Thiamin, number at (3.44 parameter (0.98 (6.14 (1.28 cm). interaction significantly affected observed parameters, namely (giving 1.71 (ea).